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HighYield T7 ARCA mRNA Synthesis Kit
| 貨號(hào) | RNT-102-L | 售價(jià)（元） | 8175 |
| 規(guī)格 | 50T x 20 μl | CAS號(hào) |
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產(chǎn)品信息
|
品牌 |
貨號(hào) |
名稱 |
規(guī)格 |
單價(jià) |
|
Jena Bioscience |
RNT-102-L |
HighYield T7 ARCA mRNA Synthesis Kit |
50T x 20 μl |
8175.00 |
HighYield T7 ARCA mRNA合成試劑盒旨在通過(guò)與T7 RNA聚合酶的體外轉(zhuǎn)錄產(chǎn)生大量ARCA帽（m）RNA。由此產(chǎn)生的5’-帽（m）RNA隨后可用于微注射、轉(zhuǎn)染或體外翻譯實(shí)驗(yàn)。與傳統(tǒng)的m7GpppG-caped（m）RNA相比，抗逆轉(zhuǎn)錄帽類似物（ARCA，m7,3'-OGpppG）caped（m）RNA具有更高的翻譯效率。這是由于m7鳥嘌呤部分的3'-O-甲基化，允許ARCA以正確（“反逆轉(zhuǎn)”）方向并入，只產(chǎn)生100%可翻譯的帶帽（m）RNA群體。
該試劑盒含有足夠的試劑，可用于15個(gè)反應(yīng)（S-Pack）或50個(gè)反應(yīng)（L-Pack）？20μL（6 mM ARCA、1.5 mM GTP、7.5 mM CTP、7.5 mL UTP、7.5 mM ATP）。使用單核苷酸格式，可以輕松實(shí)現(xiàn)ARCA濃度的單獨(dú)優(yōu)化或修飾核苷酸的合并（例如偽UTP或N4乙酰CTP）高達(dá)100%替換。培養(yǎng)30分鐘后，20μl反應(yīng)產(chǎn)生約30-50μg RNA（1μg T7對(duì)照模板，1.4 kb RNA轉(zhuǎn)錄物）。然而，產(chǎn)量可能因模板而異（啟動(dòng)子設(shè)計(jì)、序列長(zhǎng)度、二級(jí)結(jié)構(gòu)形成）。
Content:
HighYield T7 RNA Polymerase Mix
incl. RNase inhibitor and 50 % glycerol (v/v)
#RNT-102-S: 2x 40 μl
#RNT-102-L: 3x 40 μl
HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based
ATP - Solution
1x 100 μl (100 mM)
GTP - Solution
1x 100 μl (100 mM)
CTP - Solution
1x 100 μl (100 mM)
UTP - Solution
1x 100 μl (100 mM)
ARCA - Solution
#RNT-102-S: 2x 10 μl (100 mM)
#RNT-102-L: 6x 10 μl (100 mM)
T7 control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor
resulting in ~1400 nt RNA transcript
PCR-grade water
1x 1.2 ml
DTT
1x 100 μl (100 mM)
To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I
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品牌 |
貨號(hào) |
名稱 |
規(guī)格 |
單價(jià) |
|
Jena Bioscience |
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15reactions x 20 μl |
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|
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50reactions x 20 μl |
8925.00 |
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50reactions |
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HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
7875.00 |
|
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RNT-103 |
HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP) |
15reactions x 20 μl |
8805.00 |
|
Jena Bioscience |
RNT-102-S |
HighYield T7 ARCA mRNA Synthesis Kit |
15reactions x 20 μl |
4125.00 |
|
Jena Bioscience |
RNT-102-L |
HighYield T7 ARCA mRNA Synthesis Kit |
50reactions x 20 μl |
8175.00 |
|
Jena Bioscience |
RNT-101-FAMX |
HighYield T7 Fluorescein RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-DIGX |
HighYield T7 Digoxigenin RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-CY5 |
HighYield T7 Cy5 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-CY3 |
HighYield T7 Cy3 RNA Labeling Kit |
20reactions x 20 μl |
4875.00 |
|
Jena Bioscience |
RNT-101-BIOX |
HighYield T7 Biotin11 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-BIO16 |
HighYield T7 Biotin16 RNA Labeling Kit |
30reactions x 20 μl |
4425.00 |
|
Jena Bioscience |
RNT-101-AZ |
HighYield T7 Azide RNA Labeling Kit |
25reactions x 20 μl |
3975.00 |
|
Jena Bioscience |
RNT-101-AF647 |
HighYield T7 AF647 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF594 |
HighYield T7 AF594 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF555 |
HighYield T7 AF555 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-AF488 |
HighYield T7 AF488 RNA Labeling Kit |
20reactions x 20 μl |
5475.00 |
|
Jena Bioscience |
RNT-101-488-S |
HighYield T7 Atto488 RNA Labeling Kit |
20reactions x 20 μl |
5925.00 |
|
Jena Bioscience |
RNT-101-488-L |
HighYield T7 Atto488 RNA Labeling Kit |
50reactions x 20 μl |
13755.00 |
|
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RNT-101 |
HighYield T7 RNA Synthesis Kit |
50reactions x 20 μl |
2925.00 |