国产中文字幕在线观看,国产午夜精品一区二区,躁BBB躁BBB躁BBBBBB

熱銷產(chǎn)品

團(tuán)購專區(qū)

試用專區(qū)

當(dāng)前位置：首頁 - 產(chǎn)品專區(qū) - 熱銷產(chǎn)品

HighYield T7 Fluorescein RNA Labeling Kit

貨號	RNT-101-FAMX	售價（元）	4875
規(guī)格	20Tx 20 μl	CAS號

產(chǎn)品簡介
相關(guān)產(chǎn)品

產(chǎn)品信息

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-101-FAMX	HighYield T7 Fluorescein RNA Labeling Kit	20Tx 20 μl	4875.00

產(chǎn)品簡介

HighYield T7熒光素RNA標(biāo)記試劑盒設(shè)計用于通過體外轉(zhuǎn)錄產(chǎn)生隨機(jī)熒光素修飾的RNA探針。這種探針非常適合原位雜交和Northern Blot實驗。標(biāo)記原理類似于熒光素RNA標(biāo)記混合物（羅氏）的基本標(biāo)記原理。熒光素-12-UTP通過優(yōu)化反應(yīng)緩沖液和T7 RNA標(biāo)記聚合酶混合物有效地并入RNA中，作為其天然對應(yīng)物UTP的替代物。35%熒光素-12-UTP替代通常會在反應(yīng)和標(biāo)記效率之間實現(xiàn)最佳平衡。然而，熒光素-12-UTP/UTP比值的個體優(yōu)化可以通過單核苷酸格式輕松實現(xiàn)。由此產(chǎn)生的熒光素修飾RNA探針隨后可通過熒光光譜檢測。

試劑盒包含足夠的試劑，用于20個標(biāo)記反應(yīng)，每個反應(yīng)20μl（35%熒光素-12-UTP替代物、2.5 mM ATP、GTP、CTP、0.2 mM UTP、0.1 mM熒光素12-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

Fluorescein-12-UTP
1x 10 μl (5 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

相關(guān)產(chǎn)品

品牌	貨號	名稱	規(guī)格	單價
Jena Bioscience	RNT-106-S	HighYield T7 mRNA Synthesis Kit (Ψ-UTP)	15reactions x 20 μl	4425.00
Jena Bioscience	RNT-106-L	HighYield T7 mRNA Synthesis Kit (Ψ-UTP)	50reactions x 20 μl	8925.00
Jena Bioscience	RNT-105	HighYield T7 sgRNA Synthesis Kit (SpCas9)	50reactions	4425.00
Jena Bioscience	RNT-104	HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP)	15reactions x 20 μl	7875.00
Jena Bioscience	RNT-103	HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP)	15reactions x 20 μl	8805.00
Jena Bioscience	RNT-102-S	HighYield T7 ARCA mRNA Synthesis Kit	15reactions x 20 μl	4125.00
Jena Bioscience	RNT-102-L	HighYield T7 ARCA mRNA Synthesis Kit	50reactions x 20 μl	8175.00
Jena Bioscience	RNT-101-FAMX	HighYield T7 Fluorescein RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-DIGX	HighYield T7 Digoxigenin RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-CY5	HighYield T7 Cy5 RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-CY3	HighYield T7 Cy3 RNA Labeling Kit	20reactions x 20 μl	4875.00
Jena Bioscience	RNT-101-BIOX	HighYield T7 Biotin11 RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-BIO16	HighYield T7 Biotin16 RNA Labeling Kit	30reactions x 20 μl	4425.00
Jena Bioscience	RNT-101-AZ	HighYield T7 Azide RNA Labeling Kit	25reactions x 20 μl	3975.00
Jena Bioscience	RNT-101-AF647	HighYield T7 AF647 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF594	HighYield T7 AF594 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF555	HighYield T7 AF555 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-AF488	HighYield T7 AF488 RNA Labeling Kit	20reactions x 20 μl	5475.00
Jena Bioscience	RNT-101-488-S	HighYield T7 Atto488 RNA Labeling Kit	20reactions x 20 μl	5925.00
Jena Bioscience	RNT-101-488-L	HighYield T7 Atto488 RNA Labeling Kit	50reactions x 20 μl	13755.00
Jena Bioscience	RNT-101	HighYield T7 RNA Synthesis Kit	50reactions x 20 μl	2925.00

上一頁： HighYield T7 ARCA mRNA Synthesis Kit
下一頁： HighYield T7 Digoxigenin RNA Labeling Kit